Figure 3
From: Osteopontin facilitates tumor metastasis by regulating epithelial–mesenchymal plasticity
miR-429 has a crucial role in iOPN-induced MET. (a and b) Relative expression levels of miR-200 family in Hep3B, HCCLM3, and 293T cells transfected with denoted lentivirus. (c and d) Immunoblot and immunofluorescence analysis of E-cadherin and Vimentin in Hep3B cells simultaneously transfected with indicated lentivirus and miR-429 mimics or inhibitor. Scale bars, 10 μm. (e) Fold-change in mRNA levels of EMT-related transcriptional factors ZEB1 and ZEB2 in Hep3B cells treated as represented. Data represent mean±S.D. *P<0.05, **P<0.01
