Figure 2

Expression of BCL-2 family members, p53, MDM2 and p19-ARF in lymphomas from Eμ-Myc mice with CD19-Cre-mediated deletion of Mcl-1. (a) The levels of the indicated proteins were determined by Western blot analysis in three lymphomas from each of the indicated genotypes. The protein from the Mcl-1^fl allele is slightly larger than the wt MCL-1 protein indicated by arrows.⁵³ Probing for HSP70 was used as a loading control. (b) Genotype analysis of FACS-sorted B220⁺ pre-B/B-lymphoma cells from mice of the indicated genotypes, using primers that recognize both the Mcl-1^wt and Mcl-1^fl alleles, or detect the Cre recombinase transgene or the Eμ-Myc transgene. (c) Flow cytometric analysis of human CD4 reporter expression in lymphomas that arose in Eμ-Myc (negative control), Eμ-Myc;CD19-Cre;Mcl-1^fl/+ or Eμ-Myc;CD19-Cre;Mcl-1^fl/fl mice