Figure 4 | Cell Death & Disease

Figure 4

From: Hypericin-mediated sonodynamic therapy induces autophagy and decreases lipids in THP-1 macrophage by promoting ROS-dependent nuclear translocation of TFEB

Figure 4

ROS-dependent TFEB nuclear translocation activates autophagy, promoted lysosome regeneration and enhanced lipid catabolism in macrophage following HY-SDT. (a) Western blot analysis of subcellular distribution of TFEB in macrophage between nucleus and cytoplasm at specific time following HY-SDT. (b and c) Western blot and immunofluorescence analysis of the effects of 3-MA and NAC on TFEB translocation in macrophage at 2 h following HY-SDT. Scale bar=20 μm. (d) Representative western blots of TFEB following siRNA treatment. (e) Western blot analysis of TFEB effect on the activation of macrophage autophagy signaling pathway-related proteins. (f) Immunofluorescence analysis of autophagy activation in macrophage transfected with Lentivirus expressing LC3 in the presence of TFEB siRNA at 3 h following HY-SDT. Scale bar=20 μm. (g) Immunofluorescence analysis of TFEB effect on lysosomal dysfunction in macrophage as assessed Bodipy (green) and Lamp2 (red) staining at 3 h and 6 h following HY-SDT. Scale bar=20 μm. *P<0.05, **P<0.01, ***P<0.001 versus control, #P<0.05 versus SDT. All values are given as mean±S.D. (error bars) of three independent experiments

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