Figure 6 | Cell Death & Disease

Figure 6

From: Hypericin-mediated sonodynamic therapy induces autophagy and decreases lipids in THP-1 macrophage by promoting ROS-dependent nuclear translocation of TFEB

Figure 6

HY-SDT promotes the lipid efflux through ABCA1 and inhibits lipid uptake by downregulating CD36 and SR-A in macrophage. (a) Western blot analysis of cholesterol efflux transporters ABCA1, ABCG1 and SR-B1 of macrophage at 6 h after different treatments. (b) Western blot analysis of cholesterol efflux transporters of macrophage at indicated time following HY-SDT. (c) Western blot analysis of cholesterol efflux transporters of macrophage pre-treatment with CC, ATG5 siRNA and TFEB siRNA at 6 h following HY-SDT. (d) RT-PCR analysis of mRNA expression levels of cholesterol efflux transporters in macrophage pre-treatment with CC, ATG5 siRNA and TFEB siRNA at 6 h following HY-SDT. (e) Cholesterol efflux to HDL (50 μg/well) in macrophage at 6 h following HY-SDT in the presence of ABCA1 siRNA. (f) Fluorescence microscopy analysis of the uptake of DIL-ox-LDL in macrophage in the presence of CD36 siRNA and SR-A siRNA. Scale bar=100 μm. (g) RT-PCR analysis of mRNA expression levels of cholesterol uptake transporters pre-treatment with CC, ATG5 siRNA and TFEB siRNA at 6 h following HY-SDT. *P<0.05, ***P<0.001 versus control, #P<0.05 versus SDT. All values are given as mean±S.D. (error bars) of three independent experiments

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