Table 1 Signature peptides and SRM transitions for SRM analysis of SMAD3 and BRD4

From: Coordinate activities of BRD4 and CDK9 in the transcriptional elongation complex are required for TGFβ-induced Nox4 expression and myofibroblast transdifferentiation

Protein

SRM peptide sequence

Precursor m/z

Product m/z

CE (V)

Precursor Z

Product Z

Ion type

SMAD3

VETPVLPPVLVPR

708.434

777.497

27

2

1

y7

   

890.582

27

2

1

y8

   

989.650

27

2

1

y9

   

1086.70

27

2

1

y10

 

FC[Carboxyamidomethyl]LGLLSNVNR

646.842

815.473

25

2

1

y7

   

702.389

25

2

1

y6

   

872.494

25

2

1

y8

   

985.578

25

2

1

y9

 

GLPHVIYC[Carboxyamidomethyl]R

557.795

710.365

22

2

1

y5

   

847.424

22

2

1

y6

   

944.477

22

2

1

y7

   

1057.561

22

2

1

y8

BRD4

AASVVQPQPLVVVK

717.938

1106.693

28

2

1

y10

   

782.513

28

2

1

y7

   

879.566

28

2

1

y8

   

1007.624

28

2

1

y9

 

DAQEFGADVR

554.257

664.341

22

2

1

y6

   

793.383

22

2

1

y7

   

921.442

22

2

1

y8

  1. Abbreviations: m/z, mass-to-charge ratio; Z, charge state
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