Figure 1
From: Enhanced expression of Survivin has distinct roles in adipocyte homeostasis
Survivin is induced in mature adipocytes from murine white adipose tissue by HFD feeding. Survivin expression in the subcutaneous and epididymal fat of 8-week-old mice was determined by qRT-PCR (a) and western blot (b); lung tumor tissues from nude mice used as a positive control. The SVF and adipocyte fraction (AF) were divided from the subcutaneous adipose tissue (SAT) and epididymal adipose tissue (EAT) of 8-week-old mice, then protein levels of Survivin in the SVF and adipocyte fraction divided from SAT (c) and EAT (d) were respectively determined. (e–j) C57BL/6 mice were fed with HFD or standard chow diet (CD) for 24 weeks; expression levels of Survivin in SAT and EAT were determined by qRT-PCR (e) and western blot (f). The SVF and adipocyte fractions were divided from SAT and EAT respectively, then the mRNA and protein content of Survivin in the SVF and adipocyte fraction divided from SAT (g and h) and EAT (i and j) was determined, respectively. (k) Microarray analysis of GSE27017, relative expression of Survivin in 6 months HFD and 6 months old ob/ob mice. *P<0.05, **P<0.01, ***P<0.001
