Figure 2
From: MicroRNA-494 inhibits breast cancer progression by directly targeting PAK1
Overexpression of miR-494 suppresses proliferation, colony-forming, migration and invasion of breast cancer cells in vitro. (a) Growth curves of MDA-231-LUC and BT-549 cells after transfection of miR-NC or miR-494 mimics for 48 h. (b) Representative images of colony-forming ability in MDA-231-LUC and BT-549 cells after transiently transfection. (c) Wound healing assay of MDA-231-LUC and BT-549 after transfection of miR-NC or miR-494 mimics. Representative images depicting the beginning (t=0 h) and the end (t=24 h) of the recording are shown. (d) Transwell migration assay measuring MDA-231-LUC and BT-549 transfected with miR-NC or miR-494 mimics, respectively. Cell migration was analyzed 18 h after seeding in Transwells. (e) Invasion ability of MDA-231-LUC and BT-549 transfected with miR-NC or miR-494 mimics. Cell invasion was analyzed 18 h after seeding in Transwells. Data are representative of three independent experiments. Bar graphs show means of three experiments±S.D. The symbols *, ** and *** represent great significant difference (P<0.05, P<0.01 and P<0.001) by two-tailed Student’s t-test
