Figure 5
From: MicroRNA-494 inhibits breast cancer progression by directly targeting PAK1
Dysfunction of PAK1 simulates miR-494 mediated biological behaviors in MDA-231-LUC cells. (a) Western blot assay shows the efficiency of RNAi against PAK1 in MDA-231-LUC cells, with β-actin as loading control. (b) Effect of siPAK1 on the proliferation of MDA-231-LUC cells. (c) Representative images of colony-forming ability in MDA-231-LUC cells with the transfection of siPAK1. (d) Representative images of Transwell migration assay of MDA-231-LUC cells with the transfection of siPAK1. Data are representative of three independent experiments. Bar graphs show means of three experiments±S.D. The symbols *, ** and *** represent great significant difference (P<0.05, P<0.01 and P<0.001) by two-tailed Student’s t-test
