Figure 6
From: MicroRNA-494 inhibits breast cancer progression by directly targeting PAK1
PAK1 re-expression attenuates miR-494 mediated inhibition of cell proliferation, colony formation, migration and invasion in MDA-231-LUC cells. (a) Western blot analyzing the expression of PAK1 in MDA-231-LUC cells with the co-transfection of miR-NC or miR-494 together with either pcDNA3.1-EV or pcDNA3.1-PAK1. (b) Growth curves of MDA-231-LUC cells with the co-transfection of miR-NC or miR-494 together with either pcDNA3.1-EV or pcDNA3.1-PAK1. (c) Colony formation ability assays, (d) Transwell migration assays and (e) Matrigel invasion assays of the MDA-231-LUC cells with the co-transfection of miR-NC or miR-494 together with either pcDNA3.1-EV or pcDNA3.1-PAK1. Data are representative of three independent experiments. Bar graphs show means of three experiments±S.D. The symbols *, ** and *** represent great significant difference (P<0.05, P<0.01 and P<0.001) by two-tailed Student’s t-test
