Figure 1
From: Rab8a/Rab11a regulate intercellular communications between neural cells via tunneling nanotubes
Characteristics and composition of TNTs in SCs. (a) TNTs were observed between SCs in semi-confluent cultures (left: phase contrast). The morphologies of TNTs were investigated by DIC microscope (center) and SEM (right). The arrow indicates a TNT. Higher magnification images are shown in boxed areas. TNTs with characteristic bulges represented transported cargo. (b) De novo formation of TNTs by time-lapse imaging. Selected frames of a video sequence (Supplementary Movies S1) are shown. The arrow indicates the object translocation. (c) TNTs contain both F-actin and a-tubulin. Fixed SCs were immunostained with an antibody against α-tubulin (green) and phalloidin-rhodamine (red), and hoechst (blue). (d) F-actin-disrupting agent latrunculin A-blocked TNT induction in SCs. Scale bars, 100 μm, 20 μm, 50 μm (a); 20 μm (b); 20 μm(c); 50 μm (d)
