Figure 3
From: Rab8a/Rab11a regulate intercellular communications between neural cells via tunneling nanotubes
Newly synthesized RNA is transferred from SCs to axons after sciatic nerve transaction via TNTs. (a) Injured nerves showed increased formation of TNTs by F-actin staining. (b) Experimental procedure for BrU labeling. (c) Single confocal planes of fibers at nodes of Ranvier showing BrU incorporation (green) and F-actin (red). Negative control incubated with medium without BrU and 1.8 mg/ml latrunculin A during BrU labeling. Scale bars, 5 μm. (d) Relative BrU fluorescence intensities for the normal, injured and latrunculin A treated nerves, representing RNA in the axon. (n=3) Data represented mean±S.E., *P<0.05 injured group compared with control groups. **P<0.01 latrunculin A group compared with injured groups
