Figure 8

Nedd4-2 regulates the SGK/PKC pathway. (a) Western blotting showing that Nedd4-2 knockdown increases p-SGK expression and decreases p-PKC expression in the midbrain of MPTP-treated mice. Results are expressed as the mean±S.E. One-way ANOVA. **P<0.01, *P<0.05, ^##P<0.01, *represents a significant difference between other group and ctrl shRNA group; while ^#represents a significant difference between the indicated group and MPTP+ctrl shRNA group. n=3 per group. (b) Western blotting showing that PKC inhibitor increased GLT-1 and GLAST expression in MPP⁺-treated astrocytes. Results are expressed as the mean±S.E. of at least three experiments. One-way ANOVA. **P<0.01, *P<0.05, ^#P<0.05. *Represents a significant difference between other group and control group; while ^#represents a significant difference between the indicated group and MPP⁺-treated astrocytes for 24 h. (c) Model for Nedd4-2 signaling in PD