Figure 4 | Cell Death & Disease

Figure 4

From: Curcumin represses mouse 3T3-L1 cell adipogenic differentiation via inhibiting miR-17-5p and stimulating the Wnt signalling pathway effector Tcf7l2

Figure 4

The repressive effect of TCF7L2 over-expression on adipogenic differentiation can be blocked by miR-17 over-expression. (a) Illustration of the differentiation procedure with virus infection. (b–d) Detection of miR-17-5p (b), endogenous Tcf7l2 mRNA (c), as well as exogenous and endogenous TCF7L2/Tcf7l2 (d) in indicated virus infected 3T3-L1 cells 24 h after virus infection. (e–f) Oil red O staining and semi-quantitative assessment of Oil red O content. (g) Detection of five genes that encode adipogenic differentiation markers in differentiated 3T3-L1 cells infected with the correspondent virus. N> or =3 for panels (b–c) and (e–g). *P<0.05; **P<0.01; ***P<0.001; miR-17, miR-17-5p

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