Figure 5 | Cell Death & Disease

Figure 5

From: Curcumin represses mouse 3T3-L1 cell adipogenic differentiation via inhibiting miR-17-5p and stimulating the Wnt signalling pathway effector Tcf7l2

Figure 5

Curcumin represses 3T3-L1 cell differentiation, associated with the repression of miR-17-5p and the stimulation of Tcf7l2. (a–b) Curcumin treatment (with indicated dosage for 6 h) repressed miR-17 precursor and mature miR-17-5p. (c–d) Tcf7l2 mRNA and protein levels were elevated in undifferentiated 3T3-L1 cells after 6-h curcumin treatment. (e) Curcumin treatment stimulated β-cat Ser675 phosphorylation in undifferentiated 3T3-L1 cells. (f) Curcumin treatment increased Axin2 expression in 3T3-L1 cells. (g) Illustration of the differentiation procedure with curcumin treatment. (h–i) Oil red O staining and semi-quantitative assessment of Oil red O content. (j) Triglyceride levels in differentiated 3T3-L1 cells with and without curcumin treatment. (k) Tcf7l2 protein levels in un-differentiated or differentiated 3T3-L1 cells after the treatment with indicated dosages of curcumin for 24 h. N> or =3 for panels (a–c), (f), (h–j). *P<0.05 **P<0.01; ***P<0.001. CON, control; CUR, curcumin; DM, differentiation medium only; miR-17, miR-17-5p

Back to article page