Figure 1 | Cell Death & Disease

Figure 1

From: NLRP3 inflammasome activation mediates radiation-induced pyroptosis in bone marrow-derived macrophages

Figure 1

BMDM exposed to radiation shows an increased proportion of pyroptosis. Primarily cultured BMDM were exposed to 5, 10 and 20 Gy radiation, respectively. (a) Dose-dependent effects of radiation on the viability of BMDM. (b) LDH activity in supernatant was assessed by LDH Cytotoxicity Assay Kit, OD values of 490 nm were present with histogram. (c) Detected double positivity of Caspase-1 fluorescent inhibitor probe (FAM-YVAD-FMK) and PI (Q3) using flow cytometry after 24 h. The proportions of Q3 were then presented with histogram. (d) Representative flow cytometry scatter plots. Bars represent mean±S.E.M. (n=6 or 12). **P<0.001 versus 0 Gy group; #P<0.01, ##P<0.001

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