Figure 3 | Cell Death & Disease

Figure 3

From: NLRP3 inflammasome activation mediates radiation-induced pyroptosis in bone marrow-derived macrophages

Figure 3

Radiation activates Caspase-1 in BMDM in a dose-dependent pattern. Three hours after radiation, cleared lysates of BMDM were analyzed by Western Blot. (a) Immunoblot analysis of NLRP3 inflammasome-related proteins. Relative protein levels of NLRP3 (b), Caspase-1 (c), pro-IL-1β (d) and cleaved-Caspase-1 (e). Band intensities were then quantified by ImageJ software and the values of the target proteins were normalized to that of β-actin. The results are representative of three independent experiments. Bars represent mean±S.E.M. *P<0.001

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