Figure 4 | Cell Death & Disease

Figure 4

From: NLRP3 inflammasome activation mediates radiation-induced pyroptosis in bone marrow-derived macrophages

Figure 4

Nlrp3 knock out inhibited radiation-induced BMDM pyroptosis. BMDM was isolated from Nlrp3−/− mice and cultured and then exposed to 10 Gy radiation. (a) Cell viability was evaluated 24 h after radiation. (b) Supernatant LDH activity was assessed and OD values of 490 nm were presented with histogram. (c) Detected double positivity of Caspase-1 fluorescent inhibitor probe (FAM-YVAD-FMK) and PI (Q3) using flow cytometry after 24 h. The proportions of Q3 were then presented with histogram. (d) Representative flow cytometry scatter plots. Bars represent mean±S.E.M. (n=6). *P<0.05, ***P<0.0001 versus 0 Gy group; ##P<0.01, ###P<0.001

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