Figure 5 | Cell Death & Disease

Figure 5

From: NLRP3 inflammasome activation mediates radiation-induced pyroptosis in bone marrow-derived macrophages

Figure 5

Nlrp3 knock out inhibited radiation-induced BMDM Caspase-1 activation. (a) Western Blot analysis of NLRP3 inflammasome-related proteins. Relative protein levels of NLRP3 (b), Caspase-1 (c), pro-IL-1β (d) and cleaved-Caspase-1 (e). Band intensities were then quantified by ImageJ software and the values of the target proteins were normalized to that of β-actin. The results are representative of three independent experiments. (f) Concentration of IL-1β in supernatant. Bars represent mean±S.E.M. (n=6). *P<0.05, **P<0.01, ***P<0.0001 versus 0 Gy WT group; ##P<0.01, ###P<0.001

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