Figure 7
Immunofluorescence staining of DAPI (blue) and cleaved-Caspase-1 (p10, green) in mice spleens 3 h after radiation. (a) Representative microscopy images: Red arrows in magnified graph denote the fluorescence of cleaved-Caspase-1 (p10). Dotted lines indicate the border of areas; MZ, marginal zone; WP, white pulp. The results are representative of three independent experiments. Bars indicate scale of 50 μm. (b) Cleaved-Caspase-1 (p10) fluorescence was further quantified and presented as integrated optical density (IOD). Bars represent mean±S.E.M. (n=6). *P<0.001 versus 0 Gy WT group; #P<0.001
