Figure 2 | Cell Death & Disease

Figure 2

From: Loss of GCN5 leads to increased neuronal apoptosis by upregulating E2F1- and Egr-1-dependent BH3-only protein Bim

Figure 2

Knockdown of GCN5 by siRNAs induces neuronal apoptosis. (a) Rat C6 glioma cells transfected with NC (non-targeted control siRNA), siGCN5a or siGCN5b were subjected to WB to detect the GCN5 expression. Tubulin was reprobed to verify equal loadings. (b) DIV5 CGNs transfected with siGCN5a and siGCN5b together with pCMV-Gal plasmids were subjected to immunofluorescence and photos were taken on confocal microscope (scale bar=5 μm). β-Gal was stained to mark the transfected cells and the efficiency of RNA interference was determined by scoring the percentage of stained β-Gal-positive neuron population with GCN5. (c) CGNs transfected with siGCN5a and siGCN5b together with pCMV-EGFP plasmids were subjected to nucleic staining (scale bar=20 μm), and the apoptotic rate was determined by scoring the percentage of GFP-positive neuron population with nuclear pyknosis. Mean±S.E.M., n=3, *P<0.05. GFP, green fluorescent protein

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