Figure 4
Knockdown of Bim rescues the loss of GCN5-induced apoptosis. (a and b) CGNs treated with CPTH2 at the indicated doses or 50 μM in 25K for 24 h were subjected to WB or RT-PCR to detect Bcl-2, Mcl-1 or Bcl-XL expression. (c) Rat C6 glioma cells transfected with NC, siBima and siBimb were subjected to WB to detect Bim expression. (d) CGNs were transfected with NC, siBima and siBimb together with pCMV-Gal. Forty-eight hours later, neurons were exposed to CPTH2 (50 μM) in 25K media for 24 h and subjected to immunofluorescence to detect Bim expression (scale bar=5 μm). (e) CGNs were transfected with NC, siBima and siBimb together with pCMV-EGFP. Forty-eight hours later, neurons were exposed to CPTH2 (50 μM) in 25K media for 24 h and subjected to nucleic staining (scale bar=20 μm) and apoptotic analysis as in Figure 2c. White arrows indicate the transfected cells. (f) CGNs transfected with NC, siBima and siBimb together with siGCN5a and pCMV-EGFP plasmids were subjected to nucleic staining and apoptotic analysis as above. Mean±S.E.M., n=3, *P<0.05. DMSO, dimethyl sulphoxide; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; GFP, green fluorescent protein
