Figure 8 | Cell Death & Disease

Figure 8

From: Loss of GCN5 leads to increased neuronal apoptosis by upregulating E2F1- and Egr-1-dependent BH3-only protein Bim

Figure 8

SAH results in a typical neuronal apoptosis, concomitant with a transcriptional upregulation of Bim, E2F1 and Egr-1 but a downregulation of Bcl-2. (a) Representative pictures of the brains are shown after surgery. In SAH rat, significant blood clots mainly distribute at the base of Willis circle and brain stem. The same part of basal cortical brain tissue was taken for tests (circled areas). (b and c) At 24 h after SAH, neurobehavioral performance or brain water content in the left or right hemisphere (L.H or R.H.) was tested. (d) TUNEL (terminal deoxinucleotidyl transferase-mediated dUTP-fluorescein nick end labelling) assay was performed to test the apoptosis rate at 24 h after SAH, scale bar=50 μm. (e–g) The active caspase 3, Bim and Egr-1 were detected by immunofluorescence at 24 h after SAH and their positive rates of all NeuN-stained cells were calculated, scale bar in panel (e)=20 μm and in panels (f and g)=50 μm. (h and i) The protein expression levels or mRNA levels of Bim, Egr-1, E2F1 and Bcl-2 were determined by WB or RT-PCR at 24 h after SAH. The values are expressed as mean±S.E.M., *P<0.05, n=6 in each group. GAPDH, glyceraldehyde 3-phosphate dehydrogenase

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