Figure 9
GCN5 loses its activity following SAH. (a) The location of GCN5 in the neurons of sham or SAH rat were determined by co-staining with GCN5 and NeuN, scale bar=20 μm. (b) The protein expression levels or mRNA levels of GCN5 were determined by WB or RT-PCR at 24 h after SAH. (c) At 24 h after SAH, the homogenized brain tissues were subjected to a histone extraction followed by WB to detect Ac-H3K9, Ac-H3K14, Ac-H3K27, H3, Ac-H4K12, H4, Ac-H2BK5 and H2B. (d and e) At 24 h after SAH, the homogenized brain tissues were subjected to IP and the partial precipitated complexes were subjected to WB and the left ones were subjected to in vitro HAT activity assay. The values are expressed as mean±S.E.M., *P<0.05, n=6 in each group
