Figure 2
From: The oncolytic peptide LTX-315 triggers immunogenic cell death
Release of ATP from cells exposed to LTX-315. U2OS cells were cultured in the indicated conditions with LTX-315, staurosporin (STS), or CCCP. Then, live cells were stained with quinacrine and immediately analyzed by fluorescence microscopy. Representative images are shown in (a). Quantitative results (means±S.D. of triplicates) are provided in (b). Furthermore, extracellular ATP was measured in the culture supernatant, as shown in (c). Asterisks indicate significant differences (unpaired Student's t-test) with respect to untreated controls. *P<0.05; **P<0.01; ***P<0.001
