Figure 5 | Cell Death & Disease

Figure 5

From: Drosophila caspase activity is required independently of apoptosis to produce active TNF/Eiger during nociceptive sensitization

Figure 5

Secretion of soluble TNF/Eiger is constitutive and is enhanced by UV irradiation. (a) S2 cell apoptotic death in control and at different doses of UV. Anti-active caspase-3 staining (red). (b) Western blot analysis of S2 cells showing full-length TNF/Eiger from cell lysate and soluble TNF/Eiger from media supernatant. Induction by Cu2+ and UV treatment are indicated. (c) Western blot analysis of whole larval extracts with anti-TNF/Eiger at different times following UV irradiation. (d) Western blot analysis of hemolymph extracts with anti-TNF/Eiger. Time after UV irradiation is indicated. (e) Thermal allodynia (38 °C probe) upon ectopic expression of either full-length or soluble TNF/Eiger in hemocytes or fat body in the absence of UV. Genotypes are indicated

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