Figure 2
From: CRB3 regulates contact inhibition by activating the Hippo pathway in mammary epithelial cells
CRB3 overexpression promotes breast cancer cell apoptosis. (a, b) Apoptosis of MCF10A and T47D cells was examined using an apoptosis assay. **P<0.01. Data are mean±S.D. (c, d) 3D cultures were used to reveal the formation of acini of MCF10A cells. The images were photographed at the indicated times. Scale bar, 50 μm.**P<0.01. Data are mean±S.D. (e) Cleaved caspase-3 (cleaved cas 3) staining was performed on 3D cultures of MCF10A cells to examine the cells undergoing apoptosis. Scale bar, 25 μm. (f) An immunoblot analysis was used to detect the expression of apoptosis-related proteins. Cas 3, caspase 3; cas 9, caspase 9
