Figure 1 | Cell Death & Disease

Figure 1

From: Porphyromonas gingivalis gingipains cause defective macrophage migration towards apoptotic cells and inhibit phagocytosis of primary apoptotic neutrophils

Figure 1

Determination of amidolytic activity and molecular weight of purified proteolytic enzymes from P. gingivalis. Strains W83 or HG66 were grown and cultures fractionated as described. Enzyme amidolytic activity was assessed (a) for Rgp activity against Bz-L-Arg-pNA (left panel) and, for Kgp activity, against Z-L-LYS-pNA (right panel) for: membrane-bound cell extract (black bar), cell-free culture supernatant (white bar) and outer membrane fractions (grey bar). Statistical tests compared differences between strains. Enzymes were purified using chromatography. (b) Compares the TLCK inhibition as a measure of cysteine protease activity in the purified gingipains. (c) SDS-PAGE electrophoresis of P. gingivalis HG66 purified Rgp and Kgp gingipain. Statistical analyses were conducted using ANOVA followed by Bonferroni post hoc-test *P<0.05, **P<0.01, ***P<0.001. Data shown are mean±S.E.M. of three independent experiments of log phase bacterial cultures grown for 24 h (O.D. 600 nm)

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