Figure 8

Tsc1 inhibits DCs apoptosis via repressing the ROS-Bim pathway. (a) ROS production of splenic DCs from the indicated mice (n=3). (b) ROS production and apoptosis of splenic DCs, treated with or without ROS scavenger NAC (5 mM) for 16 h (n=3). (c) Apoptosis of splenic DCs, treated with apocynin (10 μM) or decylubiquinone (5 μM) for 16 h (n=3). (d) ROS production of splenic DCs, 16 h after i.v. injection with or without RAPA (30 μg per mouse) or s6k inhibitor (50 μg per mouse) (n=3). (e) ROS production of splenic DCs from the indicated mice (n=3). (f) Expression of Bim, Bcl-xl, Bcl-2 in splenic DCs. (g) Expression of Bim in splenic DCs, treated with or without ROS scavenger NAC for 16 h. All mice analyzed were 6 week old. *P<0.05, **P<0.01; error bars represent S.D.; all data are representative of at least three independent experiments