Figure 2
From: The fine tuning of metabolism, autophagy and differentiation during in vitro myogenesis
Mitochondrial biogenesis is upregulated during differentiation. (a) Mitochondrial versus nuclear DNA was determined by absolute quantitative RT-PCR. (b) WB analysis was performed with anti-oxphos and anti-CoxIV antibodies on whole-protein extracts; 20 μg of total protein were loaded in each lane; l.c.=loading control. (c) Levels of mRNA transcripts of PGC1s were determined by comparative qRT-PCR. Expression levels were normalized versus two housekeeping genes, HGPRT and 18 S. In (c) and (e). the reported values are the means of three independent experiments±S.D.; the value of proliferating myoblasts (P) is set equal to 1. *P-value <0.05; **P<0.01; ***P<0.001
