Figure 4
From: The fine tuning of metabolism, autophagy and differentiation during in vitro myogenesis
Downregulation of Beclin 1 inhibits differentiation-associated autophagy and affects the fusion step. WB analysis of MHC (200 kDa) and LC3-I (18 kDa) and II (16 kDa) (a), pS6 (32 kDa) and Beclin 1 (60 kDa) (b) and p62 (62 kDa) (c) in whole-cell extracts from muscle cells either untreated (sham, Interferin only) or treated with 20 mM Beclin 1 small interfering RNAs (siBecn). The interference procedure was carried out in proliferating myoblasts that immediately after transfection were shifted in DM for different periods of time (0, 24, 48 and 72 h). A mix of ammonium chloride and leupeptin (NH4Cl/Leu, 20 mM/100 μM) was added to the medium 2 h before cell harvest as indicated; l.c.=loading control. In panel (c) (bottom), the values of the densitometric scanning of the bands of p62 versus l.c. are provided. (d) Fusion index of sham (Interferin only) and Beclin 1-silenced terminally differentiated (TD) muscle cells (mean ±S.D.)
