Table 2 Primers designed for quantitative real-time PCR

From: Downregulation of renal tubular Wnt/β-catenin signaling by Dickkopf-3 induces tubular cell death in proteinuric nephropathy

Genes

Primers

 
 

Forward

Reverse

Human DKK-3

5′-GTAAGTTTCCCCTCTGGCTTG-3′

5′-AAGCACCAGACTGTGAAGCCT-3′

Human BAX

5′-TTCTGACGGCAACTTCAACTGG-3′

5′-AGGAAGTCCAATGTCCAGCC-3′

Human BCL-2

5′-GATGGGAACACTGGTGGAGGATGG-3′

5′-TCTGGAGGGCCCACGGCAG-3′

Human β-actin

5′-TGACGTGGACATCCGCAAAG-3′

5′-CTGGAAGGTGGACAGCGAGG-3′

Mouse NGAL

5′-ACAACCAGTTCGCCATGGTA-3′

5′-AAGCGGGTGAAACGTTCCTT-3′

Mouse KIM-1

5′-GTCGTGGGTCTTCCTGTACTC-3′

5′-AAACCAGAGATTCCCACACG-3′

Mouse cystatin C

5′-TGTTTGCACCAGGAGACAGT-3′

5′-AATTGAGCAAGGCATGGCAG-3′

Mouse Bax

5′-CCCGAGCTGATCAGAACCAT-3′

5′-GGGGTCCCGAAGTAGGAGAG-3′

Mouse Bcl-2

5′-CTTTGAGTTCGGTGGGGTCA-3′

5′-AGTTCCACAAAGGCATCCCA-3′

Mouse β-actin

5′-TCCATCATGAAGTGTGACGT-3′

5′-GAGCAATGATCTTGATCTTCAT-3′

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