Figure 2

miR-141 was downregulated by BRD7 overexpression in HEK293, 5-8F and HNE1 stable cell lines. (a) Western blot using antibodies specific for BRD7 and Flag tag confirmed the exogenous BRD7 protein levels. β-Actin served as an internal control. (b) Mature miR-141 expression was detected by qRT-PCR. U6 served as an internal control. The error bars are represented as the mean±S.E.M. ***P<0.001. All experiments were performed in triplicate