Figure 3 | Cell Death & Disease

Figure 3

From: miR-141 is involved in BRD7-mediated cell proliferation and tumor formation through suppression of the PTEN/AKT pathway in nasopharyngeal carcinoma

Figure 3

BRD7 downregulated the expression of miR-141 by repressing its promoter activity in HEK293, 5-8F and HNE1 stable cell lines. (a) The expression of pri-miR-141 and (b) pre-miR-141 was determined by qRT-PCR. GAPDH and U6 served as internal controls for pri-miR-141 and pre-miR-141, respectively. (c) Drosha and Dicer protein levels were detected by western blot. β-Actin served as an internal control. (d) The dual-luciferase reporter system assay evaluated the miR-141 potential promoter activity in HEK293, 5-8F and HNE1 cell lines and (e) in BRD7-overexpressing stable cell lines, normalized to pRL-TK. (f, upper panel) A schematic diagram showing the location of the miR-141 potential promoter region on chromosome 12 and the locations of the PCR primer pairs for the ChIP assay, and (f, lower panel) ChIP-PCR analysis for binding fragments of BRD7 within the miR-141 potential promoter region. BIRC2 was used as a positive control. F1–F8 represent the eight fragments in the miR-141 potential promoter of the PCR product. (a, b, d and e) The error bars are presented as the mean±S.E.M. **P<0.01, ***P<0.001. All experiments were performed in triplicate

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