Figure 5 | Cell Death & Disease

Figure 5

From: miR-141 is involved in BRD7-mediated cell proliferation and tumor formation through suppression of the PTEN/AKT pathway in nasopharyngeal carcinoma

Figure 5

BRD7 inhibited cell proliferation and promoted apoptosis by downregulating miR-141 expression in 5-8F and HNE1 cells. (a) MTT assays of cells transfected with miR-141M or miR-NC. (b and c) Colony-forming assay images (upper panel) and quantification of colony number/well (lower panel). (d and e) Cell-cycle analysis by flow cytometry. (f and g) Annexin V-FITC and PI double staining analysis of cell apoptosis by flow cytometry. (h) Expression of the apoptosis marker c-PARP was determined by western blot. β-Actin served as an internal control. (a–g) The error bars are presented as the mean±S.E.M. Control: vector+miR-NC, BRD7: BRD7+miR-NC. **P<0.01, ***P<0.001, NS, no significance. All experiments were performed in triplicate

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