Figure 6 | Cell Death & Disease

Figure 6

From: miR-141 is involved in BRD7-mediated cell proliferation and tumor formation through suppression of the PTEN/AKT pathway in nasopharyngeal carcinoma

Figure 6

BRD7 inhibited tumor growth by downregulating miR-141 expression in vivo. (a) Images (left panel) and tumor growth curve (right panel) of the 5-8 F xenograft model in nude mice. (b) Tumor images (left panel) and weight quantification (right panel) (N=5). (c) Western blot and (d) qRT-PCR assay confirmed the expression of the BRD7 protein and of mature miR-141 in xenograft tumor tissues, respectively. β-Actin and U6 served as internal controls, respectively. (e) IHC (DAB staining) for quantification of the proliferation marker Ki-67 (left panel) and images (right panel) and (f) TUNEL (DAB staining) for quantification of apoptosis (left panel) and images (right panel). Three tumors were analyzed per group using immunohistochemistry for the molecules mentioned above. (e and f) Original magnification, × 200; the size bars represent 25 μm. (b, d, e and f) The error bars are presented as the mean±S.E.M. Control: Vector+miR-NC, BRD7: BRD7+miR-NC. *P<0.05, **P<0.01, ***P<0.001

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