Figure 7 | Cell Death & Disease

Figure 7

From: miR-141 is involved in BRD7-mediated cell proliferation and tumor formation through suppression of the PTEN/AKT pathway in nasopharyngeal carcinoma

Figure 7

BRD7 inhibited tumor growth by repressing miR-141/PTEN/AKT signaling. (a) PTEN and phosphorylated AKT protein expression levels in miR-141 mimic-transfected 5-8F and HNE1 cells were analyzed by western blotting, and (b) significantly differently expressed proteins downstream of PTEN/AKT signaling were involved in cell-cycle progression (p27, CCND1 and CDK4) and survival (cleaved-caspase-9, total-caspase-9 and p27) when miR-141 expression was restored in BRD7-overexpressing 5-8F and HNE1 stable cell lines, respectively. β-Actin served as an internal control. (c) IHC (DAB staining) for PTEN, p-AKT, p27, CCND1 and cleaved-PARP in the 5-8F xenograft model. Three tumors were analyzed per group using immunohistochemistry for the molecules mentioned above. Original magnification, × 200; the size bars represent 25 μm. Control: Vector+miR-NC, BRD7: BRD7+miR-NC. (d) A schematic map showing the tumor suppressive mechanism of BRD7 that occurs by repressing the miR-141/PTEN/AKT pathway in NPC

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