Figure 5 | Cell Death & Disease

Figure 5

From: Low levels of Caspase-3 predict favourable response to 5FU-based chemotherapy in advanced colorectal cancer: Caspase-3 inhibition as a therapeutic approach

Figure 5

Active Caspase-3 and Ki-67 do not colocalise. (a) Tumour explant cultures remained untreated or were treated with 5FU/Oxali alone or in combination with the specific Caspase-3 inhibitor, DNLD. Tissues were sectioned and double stained for immunofluorescence for active Caspase-3 (green, left panels) and Ki-67 (red, middle panels). Nuclei were stained with Hoechst (blue, overlay in right panels). Represented images from n=x–y explant cultures per treatment condition are shown (scale bar=50 μm). (b) Detail of active Caspase-3 and Ki-67 staining in a 5FU/Oxaliplatin-treated culture, showing that Ki-67 nuclear staining corresponded to the zone of proliferating epithelial progenitors of the colonic crypts, where distinct stem cell, proliferating, and differentiating compartments are localised (scale bar=50 μm)

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