Figure 1
Anp32b deficiency impairs normal cell proliferation and oncogenic transformation. (a) The body weight of 22 Anp32b +/+, 30 Anp32b +/− and 8 Anp32b −/− mice at 4 weeks of age. Data were analyzed using Mann–Whitney U-test. **P<0.01. (b) Photographs of appearance and organs of Anp32b−/− mice and Anp32b+/+ littermate at 4 months of age. (c) The weight of organs from Anp32b+/+ and Anp32b−/− mice at 4 months of age. Data are presented as mean±S.D. and significance is *P<0.05 (n=4). (d) Western blots for the indicated protein in primary Anp32b+/+ and Anp32b−/− MEF cells. (e) Proliferation of primary Anp32b+/+ and Anp32b−/− MEFs was monitored for the indicated times. Nt/N0 represents the cell number at a given time normalized to the cell number at day 0. Data are presented as mean±S.D. The experiment is representative of three separate experiments. (f) Western blots for the indicated protein in immortalized Anp32b+/+ and Anp32b−/− MEF cells. (g) Proliferation of immortalized Anp32b+/+ and Anp32b−/− MEFs was monitored for the indicated times. Data are presented as mean±S.D. (h) Immortalized MEF cells were subcutaneously injected into nude mice and the size of masses were analyzed. Data are presented as mean±S.D.
