Figure 2
Knockdown of ANP32B inhibits breast cancer cells proliferation. (a) Breast cancer BT549, MDA-231-D3H2LN cells were stably infected with shNC and sh32b, and the indicated proteins were detected by western blot with β-actin as a loading control. (b) Cell counting of shNC- and sh32b-infected BT549, MDA-231-D3H2LN cells after 2, 4 and 6 days of growth. (c) Cell viability after 6 days of growth was measured by trypan blue exclusion. Data are presented as mean± S.D. of triplicate in an independent experiment, which was repeated for more than three times. (d) The morphology of shNC- and sh32b-infected BT549 cells under phase contrast microscopy (upper). Influence of ANP32B on colony formation of BT549 cells. Representative dishes are presented (middle). The number and size of clones were calculated for each well of six-well plates and shown in the y axis in the bottom panel. Data are presented as mean± S.D. and significance is *P<0.05, **P<0.01, which was repeated for more than three times. (e) ShNC- and sh32b-infected breast cancer MDA-231-D3H2LN cells were stably transfected with empty vector (EV) and GFP-tagged ANP32B, followed by immunoblots for the indicated proteins. (f) Cell counting of shNC/EV, sh32b/EV and sh32b/GFP-ANP32B MDA-231-D3H2LN cells after 3 days of growth. Data are presented as mean± S.D. and significance is **P<0.01, which was repeated for more than three times. (g) Representative images from the morphology and colony formation of shNC/EV, sh32b/EV and sh32b/GFP-ANP32B MDA-231-D3H2LN cells
