Figure 5
Activated hMSC-derived IFN-β suppress tumorigenicity and metastasis of MDA cells. (a and b) Invasion assay; representative images (a) and total area (b) of cell invasion assay of MDA cells isolated from act hMSC coculture with IgG or IFN-β-neutralizing antibody (α-IFN-β; 20 μg/ml). (c) Representative images of cell invasion assay of MDA cells treated with recombinant human IFN-β (rhIFN-β; 100 U/ml; R&D Systems). Values are mean±S.D. n=6; ***P<0.001; one-way ANOVA. (d and e) Images (d) and weight (e) of primary tumors in the mammary fat pad of mice 6 weeks after implantation of MDA cells isolated from act hMSC coculture with IgG or IFN-β-neutralizing antibody (α-IFN-β; 20 μg/ml). n=6; **P<0.01; Student's t-test. (f) Metastasized MDA cells in lungs of animals from (d) using quantitative PCR detecting human Alu sequences. n=6. (g) Western blot analysis of PKC-α phosphorylation and expression in MDA cells from act hMSC coculture with IgG or IFN-β-neutralizing antibody (α-IFN-β; 20 μg/ml)
