Figure 5
From: Loss of BRG1 induces CRC cell senescence by regulating p53/p21 pathway
BRG1 binds to SIRT1 and regulates p53 acetylation. (a) The protein–protein networks view from STRING database showing the networks of BRG1 (SMARCA4, the gene name coding BRG1), p53, and SIRT1. (b) Co-immunoprecipitation analysis in SW48 cells using anti-SIRT1 antibodies and anti-mouse-IgG antibodies or anti-BRG1 antibodies and anti-mouse-IgG antibodies, respectively. (c) Immunofluorescence images scanned by confocal laser microscope detecting the sub-cellular location of BRG1 and SIRT1 expression levels in SW48 cells. (d) Western blot detected the expression levels of BRG1, p53, p53-K382, and p21 in SW48 Sh-Con and Sh-BRG1 cells treated with or without EX527 (1 μmol/l, 48 h). (e) SA-β-gal staining (left panel, representative images of SA-β-gal staining; scale bar: 50 μm; right panel, percentage of SA-β-gal-positive cells from three random microscopic fields) in SW48 Sh-Con and Sh-BRG1 cells treated with or without EX527 (1 μmol/l, 48 h) DOX (0.25 μmol/l, 48 h). **P<0.01
