Figure 1 | Cell Death & Disease

Figure 1

From: Cystatin SN inhibits auranofin-induced cell death by autophagic induction and ROS regulation via glutathione reductase activity in colorectal cancer

Figure 1

Upregulated expression of CST1 mRNA and protein in colon cancer tissues and effect of auranofin (AF) treatment on cell viability. (a) qPCR analysis of CST1 expression in 21 paired non-tumor colon (Normal) and colorectal tumor (Tumor) samples. (b) Immunohistochemistry assay with NC (negative control), anti-CST1 antibody and relative fold-change analysis were performed using tissue microarray chips consisting of 9 normal colons, 2 stage I, 11 stage II, 15 stage III, and 12 stage IV colorectal adenocarcinomas. (c and d) the basal level of CST1 expression in various colon cancer cell lines by qPCR (c) and western blot analysis, including relative band intensity (d). (e) WST-1 assay in colon cancer cell lines following treatment with various doses of AF for 24 h. All data shown are the mean±S.D. of three independent experiments. *P<0.05, #P<0.01 versus control

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