Figure 2 | Cell Death & Disease

Figure 2

From: Cystatin SN inhibits auranofin-induced cell death by autophagic induction and ROS regulation via glutathione reductase activity in colorectal cancer

Figure 2

High-CST1 colon cancer cell lines are resistant to AF-induced cell death. (a) Cell viability assay in LoVo, RKO, HT-29, and SW480 cells following treatment with 2.5 μM AF for various lengths of time. (b) Percentage of apoptotic cells as assessed by DAPI staining after treatment with 2.5 μM AF for 24 h. (c) AF-induced cell death as measured by Annexin V-PI staining. Each cell was treated with 2.5 μM AF for 16 h. (d) Cell death is dependent on CST1 expression, as analyzed by western blot using the apoptosis markers caspase-3, cleaved caspase-3, and PARP. All data shown are the mean±S.D. of three independent experiments. *P<0.05, #P<0.01 versus control

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