Figure 4 | Cell Death & Disease

Figure 4

From: Cystatin SN inhibits auranofin-induced cell death by autophagic induction and ROS regulation via glutathione reductase activity in colorectal cancer

Figure 4

CST1 protects against AF-induced cell death through regulation of autophagy. (a) CST1 mRNA expression and WST-1 assay of HT-29 and SW480 shCST1 knockdown cell lines following treatment with 2.5 μM AF for 24 h. (b) Western blot of apoptosis markers in shCST1 knockdown HT-29 and SW480 cells. (c) Western blot of autophagy markers was in shCST1 knockdown HT-29 and SW480 cells. (d) CST1 mRNA expression and WST-1 assay of LoVo and RKO CST1-overexpressing lines following treatment with 2.5 μM AF for 24 h. (e) Western blot of apoptosis markers in CST1-overexpressing LoVo and RKO cells. (f) Western blot of autophagy markers in CST1-overexpressing LoVo and RKO cells. All data shown are the mean±S.D. of three independent experiments. *P<0.05, #P<0.01 versus control

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