Figure 6 | Cell Death & Disease

Figure 6

From: Cystatin SN inhibits auranofin-induced cell death by autophagic induction and ROS regulation via glutathione reductase activity in colorectal cancer

Figure 6

CST1 regulates cellular GSH to protect from AF-induced oxidative stress. (a) Western blot of GR levels in LoVo, RKO, HT-29, and SW480 cells following AF treatment for various lengths of time. (b) Cellular GSH levels in LoVo and HT-29 cells with or without AF treatment. (c) GSH levels in CST1-overexpressing LoVo and RKO cells after 12 h of incubation with AF. (d) GSH levels in shCST1 knockdown HT-29 and SW480 cells after 12 h of incubation with AF. (e) Western blot of GR and TrxR1 expression in CST1-overexpressing LoVo and RKO cells and shCST1 knockdown HT-29 and SW480 cells. (f) Western blot of catalase (Cat), SOD1, and SOD2 expression in CST1-overexpressing LoVo and RKO cells and shCST1 knockdown HT-29 and SW480 cells following AF treatment for 24 h. (g) Western blot of beclin-1 and LC3B expression in RKO and HT-29 cell lines following treatment with AF and ROS scavengers NAC (1 mM), M-TEMPO (100 μM), or DPI (2.5 μM)

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