Figure 3 | Cell Death & Disease

Figure 3

From: Solute carrier family 12 member 5 promotes tumor invasion/metastasis of bladder urothelial carcinoma by enhancing NF-κB/MMP-7 signaling pathway

Figure 3Figure 3

SLC12A5 enhances the migration and invasion of BUC cells in vitro and in vivo. (a) The expression of SLC12A5 was substantially decreased in SLC12A5-ShRNA BUC cells compared with that in ShCtrl BUC cells by western blot. (b) Suppression of SLC12A5 did not alter the cellular growth rate. (c) Representative micrographs and quantification of the invasiveness of SLC12A5-ShRNA BUC cells in the transwell matrix invasion assay compared with ShCtrl BUC cells. (d) Representative micrographs of the motility of SLC12A5-ShRNA BUC cells in the wound healing assay at 0 and 48 h compared with ShCtrl BUC cells. (e) Tail vein injection of BUC cells stably expressing SLC12A5 ShRNA or control shRNA into the nude mice led to metastasis to the lung. Left panel: image of a lung 2 months after tail vein injection of ShCtrl BUC cells and SLC12A5-ShRNA BUC cells. Right panel: number of metastases in lungs of mice (n=6 per group) 2 months after tail vein injection of ShCtrl BUC cells (mean±S.E.M., 16.0±5.5 for T24-ShCtrl cells; 15.3±6.7 for BIU-ShCtrl cells) and SLC12A5-ShRNA BUC cells (mean±S.E.M., 3.8±4.0 for T24-SLC12A5-ShRNA cells; 3.8±3.1 for BIU87-SLC12A5-ShRNA cells). The nodules were examined under an anatomical microscope; *P<0.01

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