Figure 6
SLC12A5 promotes MMP-7 expression via NF-κB-dependent transcription. (a) Western blot analysis of the relative protein levels of SLC12A5, MMP-7 and NF-κB-p65 in three groups of ShCtrol, SLC12A5 ShRNA and SLC12A5 ShRNA co-treated with overexpression of NF-κB-p65 (NF-κB-p65OE) for both T24 and BIU87 stable cell lines. The overexpression of NF-κB-p65 rescued the downregulation of MMP-7 expression induced by SLC12A5 knockdown. (b and c) The decreased ability of migration and invasion regulated by SLC12A5 knockdown was rescuced by overexpression of NF-κB-p65 in both T24 and BIU87 stable cell lines. All experiments were carried out in triplicate. Histograms represent means±S.D.
