Figure 1
From: Deletion of Pdcd5 in mice led to the deficiency of placenta development and embryonic lethality
Pdcd5 knockout mice exhibited intrauterine growth restriction and embryonic lethality. (a) Schematic representation of the mouse Pdcd5 genomic structure, the LoxP-modified Pdcd5 loci and the mutant Pdcd5 structure. Boxes represent the six Pdcd5 exons. The triangles represent the LoxP sites, and primers P1, P2 and P3 are indicated for PCR genotyping. (b and c) Representative PCR results for genotyping of postnatal mice (b) and E12.5 embryos (c) derived from intercrossing between Pdcd5+/– mice. (d) The relative Pdcd5 mRNA levels were analyzed in Pdcd5+/+, Pdcd5−/+ and Pdcd5–/– embryos by RT-PCR. (e) The expression of Pdcd5 protein in Pdcd5+/+ and Pdcd5–/–embryos detected by western blot analysis. (f–h) Representative images of Pdcd5+/+ and Pdcd5–/– embryos at E15.5 (f), E13.5 (g) and E11.5 (h). (i) Pdcd5+/+ and Pdcd5–/–embryos at E13.5 were analyzed by hematoxylin and eosin (H&E) staining. (j) Representative images of Pdcd5+/+ and Pdcd5–/–placentas at E11.5 and E13.5
