Figure 7
From: Deletion of Pdcd5 in mice led to the deficiency of placenta development and embryonic lethality
Pdcd5–/– MEFs showed decreased proliferation, increased apoptosis and cell cycle arrest. (a) Cell viability was measured in Pdcd5+/+ and Pdcd5−/− MEF cells (E10.5) at indicated time using the CCK-8 assay. Data are means±S.D. of the results from three independent experiments (**P<0.01). (b) Pdcd5+/+ and Pdcd5−/− MEF cells at E10.5 were plated in glass slides and treated with EdU for 4 h, and then analyzed by immunofluorescence. Nuclei were stained with Hoechst 33342. Representative fluorescence microscopy images are shown. (c) Treated as (b), the ratio of EdU-positive cells to the total number of cells was counted in 10 visual fields and measured by the Student’s t-test. Data are means±S.D. of the results from three independent experiments (**P<0.01). (d) Pdcd5+/+ and Pdcd5−/− MEF cells at E10.5 were cultured for 12, 24 and 36 h, and then analyzed by Annexin V/propidium iodide (PI) staining and flow cytometry. (e) The cleaved caspase-3 levels in Pdcd5+/+ and Pdcd5−/− MEF cells at E10.5 were detected by western blot analysis. (f) Pdcd5+/+ and Pdcd5−/− MEF cells at E10.5 were cultured for 24 or 48 h, and then analyzed by PI staining and flow cytometry
