Figure 5

NPAS2 promotes HCC cell survival through upregulating CDC25A. (a) Cell viability was evaluated by MTS assay in HCC cells, which were stably transfected with shRNA or forced expression vector. shNPAS2, shRNA expression vector against NPAS2; shCtrl, control shRNA. CDC25A, expression vector encoding CDC25A. (b) Colony formation assay in HLE and HLF cells treated as indicated. (c) EdU incorporation assay in HCC cells treated as indicated. Scale bar, 50 μm. (d) Cell apoptosis analysis in HCC cells with a panel of treatment as indicated. Data shown are the mean±S.E.M. from three independent experiments. *P<0.05; **P<0.01